(1981) A gas-liquid solid phase peptide and protein sequenator. (1987) Protein blotting on nitrocellulose: some important aspects of the resolution and detection of antigens in complex extracts. (1983) On the electrotransfer of polypeptides from gels to nitrocellulose membranes. and Arnaud, P.(1988) Electrotransfer of proteins following polyacrylamide gel electrophoresis nitrocellulose versus nylon membranes. (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. (1986) Technical problems concerning the use of immunoblots for the detection of antinuclear antibodies. (1986) Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on the recognition of proteins on Western blots by monoclonal antibodies. (1983) Protein blotting: principles and applications. (1985) Protein and nucleic acid blotting and immunobiochemical detection. (1982) Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a positively charged membrane filter. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. (ed.) (1994) Protein Blotting: A Practical Approach, IRL, Oxford. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis.
This process is experimental and the keywords may be updated as the learning algorithm improves. These keywords were added by machine and not by the authors. Low concentrations of sample are more easily detected because they are not spread throughout the thickness of the gel but are “concentrated” on the surface also, membranes are easier to handle and manipulate. A blot allows for rapid staining and destaining protocols of the separated proteins. Once on a solid support, procedures that would otherwise proved difficult or impossible in the gel can be undertaken. The technique itself has been modified and extended over the years ( 4). ( 3) is cited many thousands of times a year. Today, the original article by Towbin et al. Continuing the geographic theme following Southern's publication of his method for the identification of specific DNA fragments ( 1) in 1975 and the introduction of Northern blotting ( 2) not long after, the technique became known as Western blotting. Protein blotting, the transfer of proteins from a separating gel onto a thin uniform support matrix, first appeared in 1979.
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